Agarose bound Lycopersicon Esculentum (Tomato) Lectin (LEL, TL)

AL-1173瓊脂糖結(jié)合番茄凝集素

搜索關(guān)鍵詞：

番茄凝集素；血管內(nèi)灌注；凝集素灌注；微血管；內(nèi)皮細胞；LEA；TL-1176 ;  VECTOR;  LEL,TL;  LAB

產(chǎn)品訂購：【VECTOR】原裝好貨，現(xiàn)貨供應(yīng)

【溫馨提示：見我司懋康生物整理的血管功能研究標(biāo)志物-番茄凝集素產(chǎn)品專題】

產(chǎn)品描述：

番茄凝集素（Tomato lectin, Lycopersicon Esculentum lectin (LEL)）,是一種非常穩(wěn)定的單亞基糖蛋白，約含有等量的蛋白和碳水化合物。據(jù)報道其糖類部分由85%半乳糖組成，在溶液中可能形成多聚體聚合物。番茄凝集素雖然和土豆凝集素、曼陀羅凝集素（DSL）和麥胚凝集素（WGA）有部分相同的特異性、但在許多多面呈現(xiàn)出du特之處。番茄凝集素LEL能夠很好地與血型糖蛋白（glycophorin）和tamm-Horsfall糖蛋白結(jié)合，能夠有效結(jié)合和標(biāo)記嚙齒類動物的血管內(nèi)皮（vascularendothelium）

保存與運輸方法：

保存：2-8℃干燥保存、有效期一年

運輸：冰袋運輸。

Overview

Features:

Matrix is heat stable, cross-linked 4% agarose beads with a molecular exlusion of about 2x107 daltons

Bead diameter ranges in size from 45-165 microns

Matrix is stable in solutions at pH 3-11 as well as many organic solvents

Immobilized lectins are prepared using affinity purified lectins

Covalent attachment preserves lectin activity and minimizes conformational changes that might result in nonspecific or hydrophobic interactions

Hydrophilic spacer arm is inserted between the lectin and the matrix

Conjugated proteins are not leached off the beads by Tris or other routinely used buffers

No residual charges present after conjugation.  This minimizes non-specific binding to the matrix

Product supplied as a 1:1 suspension in buffer

2 mg lectin/ml gel

Inhibiting/Eluting Sugar: Chitin Hydrolysate or Glycoprotein Eluting Solution (ES-5100)

More Information

Tomato lectin is a very stable single subunit glycoprotein containing about 50 percent arabinose and galactose and may form multimeric aggregates in solution. Tomato lectin, although sharing some specificities with potato lectin, Datura lectin, and wheat germ agglutinin, has been reported to be dissimilar in many respects. LEL binds well to glycophorin and Tamm-Horsfall glycoprotein and has been used effectively to label vascular endothelium in rodents.

Agarose bound* tomato lectin is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.

This coupling method provides several advantages over the traditional cyanogen bromide procedure:

Maximum carbohydrate binding activity of the coupled lectins is retained

Linkage is stable over a range of pH values

Conjugated proteins are not leached off the beads by Tris or other routinely used buffers

No residual charges are present after conjugation. This minimizes non-specific binding to the matrix.

Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.

Inhibiting/Eluting Sugar: Chitin Hydrolysate

*2 mg lectin/ml gel

注意事項：

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另我司懋康生物提供自主品牌MKbio的番茄凝集素產(chǎn)品，質(zhì)量保證，現(xiàn)貨供應(yīng)

 — —Written/Edited by V. Shallan【版權(quán)歸MKBio懋康所有】

上海懋康生物科技有限公司是一家涉足于生命科學(xué)和生物技術(shù)領(lǐng)域研究的試劑、儀器和實驗室消耗品與實驗服務(wù)工作，主要從事細胞生物學(xué)、植物學(xué)、分子生物學(xué)、免疫學(xué)、生物化學(xué)、蛋白組學(xué)。生物制藥與診斷試劑研發(fā)生產(chǎn)等領(lǐng)域。 本公司秉承“以人為本，以誠為信、合同守信"的經(jīng)營理念。堅持"品質(zhì)保障"的原則為廣大客戶提供優(yōu)質(zhì)產(chǎn)品。

瓊脂糖結(jié)合番茄凝集素 免疫細胞組織瓊脂糖結(jié)合番茄凝集素 免疫細胞組織